Protein_Domain
miniA

Part:BBa_K877000:Design

Designed by: Alie Abele, David Pohlman   Group: iGEM12_Missouri_Miners   (2012-09-29)


Mini CipA coding region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 407
    Illegal PstI site found at 1218
    Illegal AgeI site found at 1072
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The size of the original genomic sequence from Clostridium thermocellum was reduced from 7kb to ~1kb.


Source

This part was isolated from the cipA scaffoldin from Clostridium thermocellum. Two sets of primers were used - one to isolate the type 2 dockerin region (MiniA2) and another to isolate three type-one cohesin regions and a signaling peptide for export of the protein out of the cell (MiniA1). Therefore, this part does not have the cellulose binding region of C. thermocellum's cellulosome or 6 of the type one cohesin regions. This decreased the nucleotide sequence from 7kb to ~1kb.

References